Enzymatic Synthesis of Acetyl Phosphate and Formyl Phosphate by Carbamyl Phosphate Synthetase
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منابع مشابه
Enzymatic Synthesis of Acetyl Phosphate and Formyl Phosphate by Carbamyl Phosphate Synthetase.
The utilization of rarbamyl phosphate and acetyl phosphate for synthetic pruposes has been studied in thii laboratory (l-4). Frog liver carbamyl phosphate synthetaee catalyzes the synthesis of adenosine triphosphate from acetyl phosphate and adenosine diphosphate (4); it has also been briefly reported that the enzyme could synthesize ATP from formylP and ADP (5). The reverse reactions, the synt...
متن کاملEnzymatic Synthesis of Acetyl Phosphate*
In order to prove this suggestion, a study of the chemistry of acetyl phosphate was undertaken and eventually a method of determination and a simple synthesis were worked out (3). With these available, on oxidation of pyruvate the appearance of a labile organic phosphate was observed (4) which closely resembled acetyl phosphate. The present, communication reports upon t.he isolation of this int...
متن کاملA Kinetic Study of Carbamyl Phosphate Synthetase.
While it is clear from the stoichiometry of the over-all reaction (Reaction 3) that 2 moles of ATP are utilized per mole of carbamyl phosphate formed (l), equilibrium dialysis experiments indicated that only 1 mole of ATP was bound per mole of enzyme in the absence of acetylglutamate (2). In order to gain more information about the nature of ATP binding by carbamyl phosphate synthetase, kinetic...
متن کاملCarbonic-phosphoric anhydride (carboxy phosphate). Significance in catalysis and regulation of glutamine-dependent carbamyl phosphate synthetase.
Carbonic-phosphoric anhydride (carboxy phosphate) is formed on the enzyme when glutamine-dependent carbamyl phosphate synthetase (Escherichia coli) is incubated with ATP, Mg’+, and HCO,,-. Carboxy phosphate may be trapped by borohydride reduction to formate and as the trimethyl derivative by treatment with diazomethane. The rate of enzyme-bound carboxy phosphate formation is evidently extremely...
متن کاملPurification and properties of a bacterial carbamyl phosphate synthetase.
67....---.MEISTER, A., Enzymes, 6, 247 (1YW).22. MOYED, H. S., AND MAGASANIK, B., J. Biol. Chem., 226, 351(1957).bygestonOcber9,2017hp://www.jb.org/Downladedfrom Sumner M. Kalman, Patricia H. Duffield and Thomas BrzozowskiPurification and Properties of a Bacterial Carbamyl Phosphate Synthetase 1966, 241:1871-1877.<l...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1964
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91423-7